PneumoDB is a database designed to centralize a comprehensive and verified collection of Pneumocystis mtLSUrRNA sequences, allowing Pneumocystis researchers instant access to a single unified resource.
To this purpose and in order to improve and standardize a PCR-based method of Pneumocystis spp detection, we developed a simple, rapid, low cost and optimized single-round PCR targeting the Pneumocystis mtLSUrRNA gene.
The new primer pair (pH207 and pAZ102-E) designed in this PCR can also be employed in a SYBR®Green qPCR assay
We encourage all Pneumocystis researchers to supply this database because this information will greatly enhance the use of mtLSUrRNA target sequences in epidemiological investigations of Pneumocystis infection.
Submitted sequences will be regularly cross-checked by the curators.
Database principal curator: Dr Magali Chabé – Email: firstname.lastname@example.org
How to cite PneumoDB ?
An improved single-round PCR leads to rapid and highly sensitive detection
of Pneumocystis spp.
Magali Chabe; Sara Khalife; Nausicaa Gantois; Gael Even; Christophe Audebert
Medical Mycology 2014;